余豐益 博士              換至英文頁面

     (Dr. Feng-Yih Yu) 

     職稱:教授兼系主任 中山醫學大學生物醫學科學系 

      聯絡方式

   聯絡地址:中山醫學大學生物醫學科學系 台中市建國北路一段110號

   Tel: (04)2473-0022 ext11816        Fax: (04)2324-8187   

   E-mail:fengyu@csmu.edu.tw          更新日期 01-02-2017 

 

˙學經歷

˙研究計畫及方向

˙專長及教授課程

˙學術活動

˙專利與技術移轉

˙研究著作

 經歷:

  中山醫學大學生物醫學科學系教授兼系主任,2011. 8~ 

  中山醫學大學生物醫學科學系教授,2006. 6~2011.7

  中山醫學大學生物醫學科學系副教授兼出版組組長,2005. 8~2007. 7

  中山醫學大學生命科學系副教授,2002. 8~2006.5

  中山醫學大學生命科學系助理教授,1999. 8~2002. 7

  生物技術開發中心動植物生物技術組研究員,1998-1999.

  美國威斯康辛大學食品研究所博士後研究員,1997-1998.

 

學歷:

  美國威斯康辛大學-麥迪生食品微生物及毒理學博士,1992-1997.

  台灣大學園藝研究所園產品處理組碩士,1988-1990.

  台灣大學園藝學系學士 ,1984-1988.

 

 近年研究計畫:  

    黴菌毒素之單株及單鏈抗體生產及快速檢測多重毒素之免疫奈米試紙開發(MOST 103-2313-B-040-002-MY3), 2014/8/1至2017/7/31

    玉米赤黴烯酮與柱孢藻毒素之單株抗體生產及奈米金快速免疫檢測試紙的開發應用(NSC-102-2313-B-040-004), 2013/8/1至2014/7/31

    口液中濫用藥物 現場即時檢驗之開發與實證 (102-2218-E-040-002-)  子計畫一主持人       

           濫用藥物專一性抗體生產與適用唾液樣品之快速奈米金免疫檢測試紙開發與應用   

    食品中致癌性亞硝胺之快速篩檢與暴露危害評估 (102-2632-B-040-001-MY3) 子計畫一主持人          

       亞硝胺之單株抗體生產與酵素免疫分析法及快速奈米金免疫檢測試紙之開發及應用    

   伏馬鐮孢毒素與軟海棉酸之單祩抗體生產及奈米金快速免疫試紙之開發 (NSC 101-2313-B-040-005), 2012/8/1 2013/7/31  

   台俄國合計畫-以 新潁的過氧化酶促進劑開發靈敏之冷光酵素免疫分析法來分析食品中的毒素 (NSC 100-2923-B-040-001-MY2)

           2011/8/1 2013/07/31  

    黃麴毒素與微囊藻毒之單株抗體之生產及奈米檢測試紙與螢光極化免疫分析法的開發應用 (NSC 99-2313-B-040-002-MY2)

            2010/8/1 2012/7/31

   台俄國合計畫-以螢光極化免疫分析及分子拓印技術快速分析環境中的致癌毒素  (NSC 98-2923-B-040-001-MY2)

           2009/8/1 2011/7/31  

   國科會產學合作計畫--黃麴毒素B1酵素免疫分析套組之開發 (NSC 98-2622-B-040-001-CC2), 2009/8/1 2010/7/31  

   黴菌毒素單株和單鏈抗體之生產及奈米檢測試劑之開發及應用 (NSC 96-2628-B-040-001-MY3), 2007/8/1 2010/7/31

  

 研究方向:

  1.  食品中黴菌毒素(mycotoxins) 黃麴毒素(Aflatoxin B1, M1)、赭麴毒素(Ochratoxin A)伏馬鎌孢毒素(Fumonisin B1)、橘黴素 (citrinin)

      玉米赤黴烯酮 (Zearalenone) 與藻類毒素如微囊藻毒 (Microcystin) 軟海綿酸(Okadaic acid), 石房蛤毒素 (Saxitoxin) 和多摩酸(Domoic acid)單株

      與多株抗體生產與奈米金粒子(Gold nanoparticles) 快速免疫層析檢驗試劑 (Immunochromatographic strip)之開發與應用

 2.   食品中致癌黴菌毒素如黃麴毒素(Aflatoxin B1, M1)、赭麴毒素(Ochratoxin A)、伏馬鎌孢毒素(Fumonisin B1)和玉米赤黴烯酮 (Zearalenone)

      單株抗體的生產與免疫檢驗試劑(ELISA kit) 之開發

 3.  肉類食品中瘦肉精 Ractopamine, Clenbuterol, Salbutamol 、抗生素如氯黴素, 氟甲氯黴素抗體生產與奈米金粒子(Gold nanoparticles) 快速免疫層析檢驗試劑之開發

 4.  中草藥中致癌馬兜鈴酸 (Aristolochic acid)之抗體生產及酵素免疫分析法之開發

 5.  利用酵素免疫分析法 (ELISA) 及高效液相層析分析法 (HPLC)分析鑑定食品、飼料及飲水中致癌毒素的含量與分佈



  專長及教授課程:

     生物技術學 (Biotechnology) 

    食品微生物及毒理學 (Food Microbiology & Toxicology)

    蛋白質化學 (Protein Chemistry)

    蛋白質檢測與分析實驗

    免疫化學技術 (Immunochemical Techniques)

    食品安全學 (Food Safety)

    普通生物學 (Biology)

 

 學術活動與榮譽:

  中山醫學大學科 技部105年度補助大專校院特殊優秀人才措施獎勵案

  中山醫學大學科 技部104年度補助大專校院特殊優秀人才措施獎勵案

  教育部101-103年度優秀人才獎勵補助 (3年)

  中山醫學大學科 技部100年度補助大專校院特殊優秀人才措施獎勵案

  中山醫學大學科 技部 99年度補助大專校院特殊優秀人才措施獎勵案

  俄羅斯莫斯科大學應邀訪問演講 2010 July.8.(visiting speaker). Production of antibody and development of

           ELISA and immunostrip for rapid detection of mycotoxins and phycotoxins. Moscow State University,

           Moscow, Russia. (台灣俄羅斯國際合計畫交流訪問)

  第二屆世界抗體大會應邀演講 2010. (Invited speaker). Antibody and nanotechnology used in rapid

             environmental toxin detection. Mar.24-26 (Beijing, China)

  行政院衛生署藥物食品檢驗局-食品中黴菌毒素檢驗方法制定審查委員(2008~2012)

  行政院環保署-飲水中微囊藻毒酵素免疫分析法制定審查委員(2010)

  國際期刊編輯/審查委員:

         Editorial Board: ISRN Toxicology (2010~)

           Analytica Chimica Acta (reviewer, 2013, 2014, 2015

           Analytical Biochemistry (reviewer, 2012, 2014, 2015, 2016)

           Biotechnology Journal 審稿 (reviewer,2016)

           Journal of Agricultural and Food Chemistry (SCI) 審稿 (reviewer, 2012, 2013, 2014, 2015, 2016 )

           Journal of Science of Food and Agriculture (SCI) 審稿 (reviewer, 2014, 2015)

           Food Chemistry (SCI) 審稿 (reviewer, 2013, 2014, 2015)

           Food control (SCI) (reviewer, 2014, 2015, 2016)

           Talanta (SCI) (reviewer, 2013, 2014, 2015)          

           Editor: Chung Shan Medical Journal (2005.8~2007.7)

利與技轉:   

  1. Inventor: Feng-Yih Yu; US patent number 8003766『Monoclonal antibody Specific to ochratoxin A』2009~2029.

  2. 勵勝生物科技公司產學合作計畫 (2014-5-20~2015-5-19)

  3. 睿嘉生物科技股份有限公司產學合作計畫 (2014-8~2015-8)

  4. 台灣尖端先進生技醫藥股份有限公司產學合作計畫  (2015-2~2015-7)

 

(A):期刊論文   

    1.  Liu, B. H.,  Chu, K. C., Yu, F. Y*. 2016. Novel monoclonal antibody-based sensitive enzyme-linked immunosorbent assay

         and rapid immunochromatographic strip for detecting aflatoxin M1 in milk.  Food Control 66:1-7(SCI)

    2.   Wu, T. S., Yang, J. J., Wang, Y. W., Yu, F. Y*., Liu, B. H*.  2016.  Mycotoxin ochratoxin A disrupts renal development via

          miR-731/prolactin receptor axis in zebrafish. Toxicology Research 5:519-529 (SCI).

    3.   Liu, J. W., Lu, C. C., Liu, B. H., Yu, F. Y.* 2016  Development of novel monoclonal antibodies-based ultrasensitive

          enzyme-linked immunosorbent assays and rapid immunochromatographic strips for aflatoxin B1 detection. Food Control

         59:700-707.(SCI) 

    4.  Tsai, H., Lu, Y. H., Liao, H. X., Wu, S. W., Yu, F. Y., Fuh, C. B. 2015. Detection of rabbit IgG by using functional magnetic

         particles and an enzyme-conjugated antibody with a homemade magnetic microplate. Chemistry Central Journal. 9: 8-14. (SCI)

    5.  Liu, B. H., Hung, T, C., Lu, C. C, Chou, H. N.,  Yu, F. Y*. 2014. Production of monoclonal antibody for okadaic acid

         and its utilization in an ultrasensitive enzyme-linked immunosorbent assay and one-step immunochromatographic strip

         Journal of Agricultural and Food Chemistry. 62:1254-1260. (SCI)

    6.  Vdovenko, M., Lu, C. C., Yu, F. Y*., Sakharov, I.  2014. Development of ultrasensitive direct chemiluminescent

          enzyme immunoassay for determination of aflatoxin M1 in milk. Food Chemistry 158:310-314. (SCI)   

    7.   Wu, T. S.,  Yang, J. J., Yu, F. Y*.,  Liu, B. H*.  2013. Cardiotoxicity of mycotoxin citrinin and involvement of

          microRNA-138 in zebrafish embryos. Toxicological Sciences 136:402-412.  (SCI)

    8.   Vdovenko, M., Hung, C. T.,. Sakharov, I., Yu, F. Y*  2013. Determination of okadaic acid in shellfish by

          using a novel chemiluminescent enzyme-linked immunosorbent assay method. Talanta 116:343-346. (SCI)  

    9.  Yu. F. Y., Gribas, A., Vdovenko, M., Wang, J. J., Sakharov, I2013.  Development of ultrasensitive direct

          chemiluminescent enzyme immunoassay for determination of aflatoxin B1 in food products.  Talanta 107:285-29. (SCI)  

   10.   Liu, B. H., Hsu, Y. T, Lu, C. C, Yu, F. Y*.  2013. Detecting aflatoxin B1 in foods and feeds by using sensitive

           rapid enzyme-linked immunosorbent assay and gold nanoparticle immunochromatographic strip.  Food Control

           30:184-189 (SCI)

   11.   Wu, T. S., Yang, J. J., Yu, F. Y*, Liu, B. H*. 2012. Evaluation of nephrotoxic effects of mycotoxins, citrinin

          and patulin on zebrafish embryos. Food Chemical Toxicology 50:4398-4404. (SCI)

   12.  Yu, F. Y., Vdovenko, M., Wang, J. J., Sakharov, I. 2011.  Comparison of enzyme-linked immunosorbent

          assays with chemiluminescent and colorimetric detection for determination of ochratoxin A in food

          Journal of Agricultural and Food Chemistry. 59:809-813.(SCI).

   13.  Wang, J. J., Liu, B. H., Hsu, Y. T., Yu, F. Y*. 2011. Sensitive enzyme-linked immunosorbent assay

          and gold  nanoparticle immunochromatographic strip for detecting aflatoxin M1 in Milk.

          Food Control. 22:954-959.(SCI) 

   14. Chang, C. H. , Yu, F. Y*., Wu, T. S. Wang, L. T., Liu, B. H.*2011. Mycotoxin citrinin induced cell cycle

        G2/M arrest and numberical chromsomal aberration associated with disruption of microtubule formation in

         human cells. Toxicological Sciences 119:84-92.(SCI)  (equal contribution to the first author)

   15. Yu, F. Y., Wu, T. S., Chen, T. W., Liu, B. H. 2011. Aristolochic acid induced oxidative DNA damage

         associated with glutathione  depletion and ERK1/2 activation in HL-60 cells.  Toxicology in vitro 25:810-816.(SCI) 

   16 . Yu, F. Y., Chu, T. Y., Lian J.D., Wu, S. W., Hung, T, W., Chang, H. R., 2011. Induction of oxidative stress

         and DNA damage in human renal proximal tubular cells by arsistolochic acid. Journal  of Food and

         Drug Analysis. (SCI) 19:114-122. 

   17.  Liu, M. C., Lin, T. H., Wu, T. S., Yu, F. Y., Lu, C. C., Liu, B. H.* 2011. Aristolochic acid I suppressed iNOS

         gene expression and NF-kappa B activation in stimulated macrophage cells. Toxicology Letters 202:93-99. SCI) (in Press)

   18. Shiu, C. M., Wang, J. J., Yu, F. Y.* 2010.  Sensitive enzyme-linked immunosorbent assay and  rapid one-step

        immunochromatographic strip for fumonisin B1 in grain based food and feed  samples.  Journal of Science of 

        Food and Agriculture 90: 1020-1026. (SCI)  

   19.  Chang,C. H. , Yu, F. Y., Lin, Y. S., Wang, L. T., Liu, B. H. 2009. Activation of ERK and JNK  signaling pathways 

         by mycotoxin citrinin in human cells. Toxicol. Appl. Pharmacol. 237 :281-287. (SCI)

   20.  Liao, Y. C., Wu, T. S., Yu, F. Y., Tsai, C. H., Liu, B. H.* 2008. Mycotoxin patulin induced apoptosis in 

          mammalian cells through a p53-independent pathway. Toxicology Letters 183:105-111. (SCI)

   21. Liu, B. H., Tsao, Z. J., Wang, J. J.,  Yu, F. Y.*  2008.  Development of a monoclonal antibody against ochratoxin A 

        and its application in enzyme-linked immunosorbent assay and gold nanoparticle immunchromatographic strip.  

        Analytical Chemistry 80: 7029-7035. (SCI, IF: 5.287)

  22. Tsao, Z. J., Liao Y. C. Liu, B. H.,  Su, C. C., Yu, F. Y.* 2007. Development of a monoclonal  antibody  

         against domoic acid and its application in enzyme-linked immunosorbent  assay  and colloidal gold immunostrip.

         Journal of Agricultural and Food Chemistry 55: 4921-4927. (SCI)

  23Liu, B. H., Wu, T. S., Yu, F. Y., Su, C. C. 2007. Induction of oxidative stress response by the

           mycotoxin patulin in mammalian cells. Toxicological Sciences 95(2):340-347. (SCI)

  24.  Yu, F. Y*., Lin, Y. H., Su, C. C. 2006.  A sensitive enzyme-linked immunosorbent assay for detecting 

          carcinogenic aristolochic acid in in herbal remedies Journal of Agricultural  and Food Chemistry 54: 2496-2501. (SCI)  

  25. Liu, B. H., Wu, T. S., Yu, F. Y., Wang, C. H. 2006. Mycotoxin patulin activates p38 and   

           JNK signaling pathways in human embryonic kidney cells. Toxicological Sciences 89: 423-430. (SCI)  

  26. Yu, F. Y.*,  Liao, Y. C., Chang, C. H., Liu, B. H  2006. Citrinin induces apoptosis in HL-   

           60 cells via mitochondrial pathway. Toxicology Letters 161:  143-151. (SCI)  

  27. Yu, F. Y.*. T-F. Chi, B. H. Liu., Su, C. C. 2005. Development of a sensitive enzyme-linked  immunosorbent 

            assay  for the  determination of  ochratoxin A. Journal of Agricultural and Food Chemistry 53: 6947-953. (SCI)

  28. Wu, T. S., Yu, F. Y., Su, M. C., , Kan, C. C., Liu, B. H.  2005. Activation of ERK mitogen-activated protein kinase

          by mycotoxin Patulin in human cells. Toxicology and Applied Pharmacology. 207:103-111. (SCI)

  29. Liu, B. H.,  T. S. Wu,  M. C. Su, C. P. Chung,  F. Y. Yu* 2005.  Evaluation of Citrinin occurrence and cytotoxicity in

          Monascus fermentation products.   Journal of Agricultural and Food Chemistry 53:170-175. (SCI)

  30. Yu, F. Y*., B. H. Liu, T. S. Wu, T-F. Chi, M. C. Su. 2004. Development of a sensitive enzyme-linked immunosorbent 

          assay  for the  determination of  domoic acid in shellfish.  Journal of Agricultural and Food Chemistry  52: 5334-5339. (SCI)

  31.  Liu, B. H., F. Y. Yu, T. S. Wu, S. Y. Li, M. C. Su, M. C. Wang, and S. M. Shih. 2003. Evaluation of genetic risk and

           oxidative DNA damage in mammalian cells exposed to mycotoxins, patulin and citrinin. Toxicology and Applied

           Pharmacology. 191: 255-263.   (SCI)

 32. Liu, B. H., C. Y. Huang, F. Y. Yu, and P. C. Lin. 2003. The presence and expression of aflR gene in various Aspergillus species. 

           Food Science and Agricultural Chemistry. 4:149-155.

 33 Yu, F. Y*., B. H. Liu, H. R. Chou, F. S. Chu. 2002. Development of a sensitive ELISA for the determination 

          of microcystins in algae. Journal of Agricultural and Food Chemistry. 50:4176- 4182.(SCI) 

 34.  Liu, B.-H*., Yu, F. Y.  M. H. Chan, and Y. L. Yang. 2002. The effects on mycotoxins,  fumonisin B1 and aflatoxin B1,

           on primary swine alveolar macrophages. Toxicology and Applied Pharmacology.180:197-204. (SCI)

 35.  Liu, B.-H., Yu, F. Y.*. 2001. DNA affinity-purified AFLR binds to the 5' upstream regions of aflatoxin pathway genes.

           Food Science and Agricultural Chemistry. 3:84-90.

 36.  Christensen, H., F.-Y. Yu, and F. S. Chu. 2000. Development of a polyclonal antibody-based sensitive Enzyme-linked

             immunosorbent assay for Fumonisin B4. Journal of Agricultural and Food Chemistry  48:1977-1984.(SCI).  

 37.  Liu, B.-H., Yu, F. Y., X. Huang, and F. S. Chu. 2000. Monitoring of microcystin- protein phosphatase adduct formation

            with immunochemical methods. Toxicon  38:619-632. (SCI).
 38. Yu, F.-Y. and F. S. Chu. 1999. Production and characterization of monoclonal antibodies against fumonisin B1.

           Food  and Agricultural Immunology 11:297-306.(SCI)
 39.  Yu, F.-Y. and F. S. Chu. 1999. Production and characterization of monoclonal anti-anti-idiotype antibodies against

            fumonisin B1. Journal of Agricultural and Food Chemistry 47:4815-4820.(SCI).
 40.  Wanjun Yu., F.-Y. Yu, D. Undersander, and F. S. Chu. 1999. Immunoassays of selected mycotoxins in hay, silage and

            mixed feed. Food and Agricultural  Immunology 11:307-319.(SCI).

 41. Yu, F.-Y. and F. S. Chu. 1998. Analysis of fumonisins and AAL toxin by Liquid Chromatography-Enzyme-Linked 

          Immunosorbent Assay. J. Assoc. Off. Anal. Chem. Int.  81:749-756. (SCI)

 42. Yu, F.-Y. and F. S. Chu. 1996. Production and characterization of antibodies against fumonisin B1. Journal of Food Protection.

         59: 992-997.(SCI)

 43.  Liu, B.-H., F.-Y. Yu, and F. S. Chu. 1996. Anti-idiotype and anti-anti-idiotype  antibodies generated from polyclonal antibodies

         against microcystin-LR. Journal of Agricultural and Food Chemistry. 44: 4037-4042. (SCI)

 

 (B) 研討會論文

     1. 
Liu, J. W., Lu, C. C., Yu, F. Y.  2014. Production of a monoclonal antibody against aflatoxin B1 and its application in enzyme-linked

          immunosorbent assay and rapid immunochromatographic strip. Presented at American Chemistry Society 248th Annual Meeting, Aug 10-14,

           2014 in San Francisco, CA. USA.     

    2.   Hung, C. T., Yu, F. Y.* 2012. Development of a monoclonal antibody against okadaic acid and its application in enzyme-linked

         immunosorbent assay and gold nanoparticle immunochromatographic strip.  Presented at 51st Annual Meeting of the Society of Toxicology,

        March 11-15, 2012  in San Francisco, CA. USA.

    3..  Yu, F. Y. (Invited speaker) 2010.  Antibody and nanotechnology used in rapid environmental toxin detection..

           2nd Annual international Congress of Antibodies. Beijing, China (中國,北京)  

    4.   Wang, J. J.,  Yu, F. Y.*   2008.  Development of  enzyme-linked immunosorbent assay and gold nanoparticle 

            immunostrip for Aflatoxin M1. Presented at American Chemistry Society 236th Annual Meeting, Aug  2008.

             Philadelphia, PA, USA.

    5.   Wang, J. J.,  Yu, F. Y.*   2008.  Development of  enzyme-linked immunosorbent assay and gold nanoparticle 

             immunostrip for Fumonisin B1.  Presented at Taiwan Biomedical  Annual  Meeting, March  2008.

    6   Tsao, Z. J., Liao Y. C. Liu, B. H.,  Su, C. C., Yu, F. Y.* 2007.  Production of monoclonal antibody against 

            ochratoxin A and  its application to enzyme-linked immunosorbent assay and colloidal gold immunostrip. 

           Presented at Taiwan Biomedical  Annual  Meeting, March 2007.     

    7. Tsao, Z. J.,  Yu, F. Y.* 2006.  Production of monoclonal antibody against domoic acid  and  its application to 

           enzyme-linked immunosorbent assay and colloidal gold immunostrip. Presented at Taiwan Algae Society Annual  Meeting, 

           Nov 2006.   

    8.  Liu, B. H., F. Y. Yu.*  2002. Production and characterization of antibodies for detection of microcystins and domoic acid.

            International symposium on harmful algae management and mitigation in the area of south China sea.  Dec. 2002. (Invited speaker)    

    9. Liu, B. H., F. Y. Yu, and T. H. Chiu. 2000. Binding of AFLR to the 5' upstream regions of aflatoxin pathway genes. 

           Presented at Taiwan Biomedical Annual Meeting, March 2000.    

    10. Yu, F.-Y. and J. F. Leslie, and F. S. Chu. 1998. Immunochemical screening of  Fusarium cultures for their ability to produce fumonisins.

           Presented at American Society for Microbiology (ASM) Annual Meeting, May 1998.


 (C) 專書及專書論文

    1. Yu, F.-Y. 1997. Immunochemical studies on fumonisins. Ph.D dissertation. 

        University of Wisconsin-Madison. Madison, Wisconsin.
 
   2. 余豐益. 1999. 免疫檢驗試劑技術原理與發展趨勢.經濟部產業技術叢書.1999 年7月出版

   3. 余豐益. 2003. 免疫診斷技術. 教育部生物技術科技改進計--分子檢驗第五章  64-84 pp.