-
Lin, E.S., Huang, C.Y.*
(2024) Binding pattern and structural interactome of the anticancer
drug 5-fluorouracil: A critical review.
Int. J. Mol. Sci., 25, 3404. (SCI)
-
Lee, C.Y., Chen, Y.C.,
Huang, Y.H., Lien, Y., Huang, C.Y.*
(2024) Cytotoxicity and multi-enzyme inhibition of Nepenthes
miranda stem extract on H838 human non-small cell lung cancer
cells and RPA32, elastase, tyrosinase, and hyaluronidase proteins.
Plants, 13, 797.
(SCI)
-
Wu, S.W., Chen, Y.J., Chang, Y.W.,
Huang, C.Y., Liu, B.H., Yu, F.Y.*
(2024) Novel enzyme‑linked aptamer‑antibody sandwich assay and hybrid
lateral flow strip for SARS‑CoV‑2 detection. J.
Nanobiotechnology, 22, 5. (SCI)
-
Huang, Y.H., Huang, C.Y.*
(2024) The complexed crystal structure of dihydropyrimidinase reveals
a potential interactive link with the neurotransmitter γ-aminobutyric
acid (GABA). Biochem. Biophys. Res. Commun.,
692, 149351. (SCI)
-
Chen, C.B., Wang, C.W., Lu, C.W., Chen, W.T., Zhou, B.R., Chu, C.Y.,
Hsu, S.F., Yang, C.T., Chang, J.W.C., Yang, C.K., Wang, C.L., Fang,
Y.F., Hsu, P.C., Hua, C.C., Wu, C.E., Ko, H.W., Chen, K.C., Yang,
Y.C., Tseng, H.C., Cheng, A.Y., Tseng, L.C., Shih, F.Y., Hung, S.I.,
Huang, C.Y.*, Chung,
W.H.* (2024) HLA-B*51:02 is associated with an increased risk
of osimertinib-induced severe hypersensitivity. J. Allergy
Clin. Immunol. Pract., in revision. (SCI)
-
Su, H.H., Huang, Y.H., Lien, Y., Yang, P.C.,
Huang, C.Y.* (2023) Crystal structure of DNA
replication protein SsbA complexed with the anticancer drug
5-fluorouracil. Int. J. Mol. Sci., 24,
14899. (SCI)
-
Lin, E.S., Huang, C.Y.*
(2023) The inhibition activities of the fruit extract of Plinia
cauliflora against melanoma cells and the single-stranded
DNA-binding protein (SSB) from Klebsiella pneumoniae.
Appl. Sci., 13, 11061. (SCI)
-
Lin, E.S., Huang, Y.H., Chung, J.C., Su, H.H.,
Huang,
C.Y.* (2023) The inhibitory
effects and cytotoxic activities of the stem extract of Nepenthes
miranda against single-stranded DNA-binding protein and oral
carcinoma cells. Plants, 12, 2188.
(SCI)
-
Chen, P.J., Lin, E.S., Su, H.H.,
Huang,
C.Y.* (2023) Cytotoxic,
antibacterial, and antioxidant activities of the leaf extract of
Sinningia bullata. Plants, 12, 859.
(SCI)
-
Huang, Y.H., Yang, P.C., Lin, E.S., Ho.Y.Y.,
Peng, W.F., Lu, H.P., Huang, C.C., Huang,
C.Y.* (2023)
Crystal structure of allantoinase from Escherichia coli BL21:
a molecular insight into a role of the active site loops in catalysis.
Molecules, 28, 827.
(SCI)
-
Lin, E.S.,
Huang, Y.H., Yang, P.C., Peng, W.F., Huang,
C.Y.* (2023)
Complexed crystal structure of the dihydroorotase domain of human CAD
protein with the anticancer drug 5-fluorouracil.
Biomolecules, 13, 149. (SCI)
-
Liu, H.W.,
Chiang, W.Y.,
Huang, Y.H., Huang,
C.Y.* (2022)
The inhibitory effects and cytotoxic activities of the stem extract of
Sarracenia purpurea against melanoma cells and the SsbA
protein.
Plants, 11, 3164. (SCI)
-
Lin, E.S., Huang,
C.Y.* (2022)
Cytotoxic activities and the allantoinase inhibitory effect of the
leaf extract of the carnivorous pitcher plant Nepenthes miranda.
Plants, 11, 2265. (SCI)
-
Huang, Y.H., Chiang, W.Y., Chen, P.J., Lin, E.S.,
Huang, C.Y.* (2022)
Anticancer and antioxidant activities of the root extract of
the carnivorous pitcher plant Sarracenia purpurea.
Plants, 11, 1668. (SCI)
-
Lin, E.S.,
Huang, Y.H., Luo, R.H., Basharat, Z., Huang,
C.Y.*
(2022) Crystal structure of an SSB protein from Salmonella enterica
and its inhibition by flavanonol taxifolin.
Int. J. Mol. Sci., 23, 4399.
(SCI)
-
Lin, E.S., Luo, R.H.,
Yang, Y.C., Huang,
C.Y.*
(2022) Molecular insights into how the dimetal center in
dihydropyrimidinase can bind the thymine antagonist 5-aminouracil: A
different binding mode from the anticancer drug 5-fluorouracil.
Bioinorg. Chem. Appl.,
2022, 1817745. (SCI)
-
Lin, E.S., Luo, R.H., Huang,
C.Y.*
(2022) A complexed crystal structure of a single-stranded DNA-binding
protein with quercetin and the structural basis of flavonol inhibition
specificity.
Int. J. Mol. Sci., 23, 588. (SCI).
-
Guan,
H.H.,
Huang,
Y.H.,
Lin, E.S.,
Chen,
C.J.,
Huang, C.Y.*
(2021) Structural analysis of Saccharomyces cerevisiae
dihydroorotase reveals molecular insights into the tetramerization
mechanism. Molecules,
26, 7249. (SCI)
-
Lin, E.S.,
Huang,
Y.H.,
Huang, C.Y.*
(2021) Characterization of the chimeric PriB-SSBc protein.
Int. J. Mol. Sci., 22, 10854. (SCI)
-
Guan,
H.H.,
Huang,
Y.H.,
Lin, E.S.,
Chen,
C.J.,
Huang, C.Y.*
(2021) Complexed crystal structure of Saccharomyces cerevisiae
dihydroorotase with inhibitor 5-fluoroorotate reveals a new binding
mode.
Bioinorg. Chem. Appl., 2021, 2572844.
(SCI)
-
Guan,
H.H.,
Huang,
Y.H.,
Lin, E.S.,
Chen,
C.J.,
Huang, C.Y.*
(2021) Plumbagin, a natural product with potent anticancer activities,
binds to and inhibits dihydroorotase, a key enzyme in pyrimidine
biosynthesis. Int. J. Mol. Sci., 22, 6861. (SCI)
-
Guan,
H.H.,
Huang,
Y.H.,
Lin, E.S.,
Chen,
C.J.,
Huang, C.Y.*
(2021) Structural basis for the interaction modes of
dihydroorotase with the anticancer drugs 5-fluorouracil and
5-aminouracil.
Biochem.
Biophys. Res. Commun.,
551, 33-37. (SCI)
-
Lin, E.S., Huang,
C.Y.* (2021)
Crystal structure of the single-stranded DNA-binding protein SsbB in
complex with the anticancer drug 5-fluorouracil: Extension of the
5-fluorouracil interactome to include the
oligonucleotide/oligosaccharide-binding fold protein.
Biochem.
Biophys. Res. Commun.,
534, 41-46. (SCI)
-
Wang CW, Tassaneeyakul W, Chen CB, Chen WT, Teng YC,
Huang CY, Sukasem C,
Lu CW, Lee YS, Choon SE, Nakkam N, Chung-Yee Hui R, Huang YH, Chang
YC, Yu-Wei Lin Y, Chang CJ, Chiu TM, Chantratita W, Konyoung P, Lee
CN, Klaewsongkram J, Rerkpattanapipat T, Amornpinyo W, Saksit N,
Rerknimitr P, Huei HY, Lin SH, Hsu CK, Fan WL, Chan CC, Lin YJ, Hung
SI, Chung WH (2021) Whole genome sequencing identifies genetic
variants associated with co-trimoxazole hypersensitivity in Asians.
J. Allergy Clin. Immunol.,
147, 1402-1412.
(SCI)
-
Huang,
Y.H.,
Huang,
C.Y.*
(2021) Comparing SSB-PriA functional and physical interactions in
Gram-positive and -negative bacteria. Methods Mol. Biol.,
2281, 67-80.
-
Huang,
C.Y.* (2020)
Structure, catalytic mechanism, posttranslational lysine
carbamylation, and inhibition of dihydropyrimidinases.
Adv. Protein Chem. Struct. Biol.
(Elsevier), 122, 63-96. (SCI)
-
Huang,
Y.H.,
Lien,
Y.,
Chen,
J.H., Lin, E.S., Huang,
C.Y.* (2020)
Identification and characterization of dihydropyrimidinase inhibited
by plumbagin isolated from Nepenthes miranda extract.
Biochimie, 171-172, 124-135. (SCI)
-
Huang, Y.H., Chen, I.C., Huang, C.Y.*
(2019) Characterization of an SSB–dT25 complex: structural insights into
the S-shaped ssDNA binding conformation.
RSC Adv.,
9, 40388-40396. (SCI)
-
Huang, Y.H., Lin, E.S., Huang, C.Y.*
(2019) Complexed crystal structure of SSB reveals a novel
single-stranded DNA binding mode (SSB)3:1: Phe60 is not
crucial for defining binding paths. Biochem. Biophys. Res.
Commun., 520, 353-358. (SCI)
-
Huang,
Y.H., Ning, Z.J.,
Huang, C.Y.*
(2019) Crystal structure of dihydropyrimidinase in
complex with anticancer drug 5-fluorouracil.
Biochem.
Biophys. Res. Commun.,
519, 160-165.
(SCI)
-
Chen, K.L.,
Huang,
Y.H., Liao, J.F., Lee, W.C.,
Huang, C.Y.*
(2019) Crystal structure of the C-terminal domain
of the primosomal DnaT protein: Insights into a new oligomerization
mechanism.
Biochem.
Biophys. Res. Commun.,
511, 1-6.
(SCI)
-
Huang,
Y.H.,
Huang, C.Y.*
(2018) The glycine-rich flexible region in SSB is crucial for PriA
stimulation. RSC Adv.,
8,
35280-35288.
(SCI)
-
Cheng, J.H.,
Huang,
Y.H.,
Lin,
J.J.,
Huang, C.Y.*
(2018)
Crystal structures of monometallic dihydropyrimidinase
and the human dihydroorotase domain K1556A mutant reveal no lysine
carbamylation within the active site.
Biochem.
Biophys. Res. Commun.,
505, 439-444.
(SCI)
-
Huang, C.Y.*
(2018) Crystal structure of SSB complexed with
inhibitor myricetin.
Biochem.
Biophys. Res. Commun.,
504, 704-708.
(SCI)
-
Chen, K.L.,
Cheng, J.H.,
Lin, C.Y.,
Huang,
Y.H.,
Huang, C.Y.*
(2018) Characterization of single-stranded DNA-binding protein SsbB
from Staphylococcus aureus: SsbB cannot stimulate PriA
helicase. RSC Adv., 8, 28367-28375. (SCI)
-
Huang,
Y.H., Huang,
C.Y.* (2018)
SAAV2152 is a single-stranded DNA binding
protein: the third SSB in Staphylococcus aureus.
Oncotarget, 9, 20239-20254. (SCI)
-
Cheng, J.H., Huang,
C.C., Huang, Y.H., Huang,
C.Y.* (2018)
Structural basis for pH-dependent oligomerization
of dihydropyrimidinase from Pseudomonas aeruginosa PAO1.
Bioinorg. Chem. Appl., 2018, 9564391.
(SCI)
-
Huang,
Y.H.,
Guan,
H.H.,
Chen,
C.J.,
Huang, C.Y.*
(2017) Staphylococcus aureus single-stranded DNA-binding
protein SsbA can bind but cannot stimulate PriA helicase.
PLoS One, 12, e0182060. (SCI)
-
Tzeng,
C.T.,
Huang,
Y.H.,
Huang,
C.Y.* (2016)
Crystal structure of dihydropyrimidinase from Pseudomonas
aeruginosa PAO1: Insights into the molecular basis of
formation of a
dimer.
Biochem.
Biophys. Res. Commun.,
478, 1449-1455. (SCI)
-
Ko, T.P., Huang, C.Y.,
Hsieh, T.J., Chen, S.C., Chen, Y.R., Yang, C.S., Kuo, H.C., Wang,
W.L., Hsiao, T.H., Lin, C.H., Chen, Y.* (2016) Crystal structures of
Staphylococcal SaeR reveal possible DNA-binding modes.
Biochem.
Biophys. Res. Commun.,
474, 686-690. (SCI)
-
Huang,
C.C., Huang,
C.Y.* (2016)
DnaT is a PriC-binding protein.
Biochem.
Biophys. Res. Commun.,
477, 988-992. (SCI)
-
Huang,
Y.H.,
Lien,
Y.,
Huang,
C.C., Huang,
C.Y.* (2016)
Characterization of Staphylococcus aureus
primosomal DnaD protein: Highly conserved C-terminal region is
crucial for ssDNA and PriA helicase binding but not for DnaA
protein-binding and self-tetramerization. PLoS One,
11, e0157593. (SCI)
-
Huang,
C.Y.* (2015)
Inhibition of a putative dihydropyrimidinase from Pseudomonas
aeruginosa PAO1 by flavonoids and substrates of cyclic
amidohydrolases. PLoS One,
10, e0127634. (SCI)
-
Huang,
Y.H., Huang,
C.Y.* (2015)
Creation of
a putative third metal binding site in type II dihydroorotases
significantly enhances enzyme activity. Protein Pept.
Lett., 22, 1117-1122.
(SCI)
-
Huang,
C.Y.*, Abe Y., Ding, H., Chung, IF. (2015) Helicase and its
interacting factors: regulation mechanism, characterization,
structure, and application for drug design. An Editorial. Biomed Res. Int., 2015,
909047. (SCI)
-
Huang,
Y.H.,
Huang,
C.C.,
Chen,
C.C.,
Yang,
K.J., Huang,
C.Y.* (2015)
Inhibition of
Staphylococcus aureus
PriA helicase by flavonol kaempferol.
Protein
J., 34, 169-172. (SCI)
-
Huang,
Y.H., Huang,
C.Y.* (2014)
Structural insight into the DNA-binding mode of the primosomal
proteins PriA, PriB, and DnaT.
A review
article. Biomed Res. Int., 2014, 195162. (SCI)
-
Huang,
Y.H., Huang,
C.Y.* (2014)
C-terminal
domain swapping of SSB changes the size of the ssDNA
binding site. Biomed Res. Int., 2014,
573936. (SCI)
-
Peng,
W.F., Huang, C.Y.* (2014) Allantoinase and
dihydroorotase binding and inhibition by flavonols and the substrates
of cyclic amidohydrolases.
Biochimie, 101, 113-122. (SCI)
-
Huang,
Y.H., Lin, M.J., Huang, C.Y.* (2013) DnaT is a
single-stranded DNA binding protein. Genes Cells, 18,
1007-1019. (SCI)
-
Huang,
Y.H., Huang, C.Y.* (2013) The N-terminal
domain of DnaT, a primosomal DNA replication protein, is crucial for
PriB binding and self-trimerization. Biochem.
Biophys. Res. Commun.,
442, 147-152. (SCI)
-
Ho,
Y.Y., Huang, Y.H., Huang, C.Y.* (2013) Chemical rescue
of the post-translationally carboxylated lysine mutant of allantoinase
and dihydroorotase by metal ions and short-chain carboxylic acids. Amino
Acids, 44, 1181-1191. (SCI)
-
Huang,
Y.H., Lin, M.J., Huang, C.Y.* (2013) Yeast two-hybrid analysis of
PriB-interacting proteins in replication restart primosome: a proposed
PriB-SSB interaction model. Protein
J., 32, 477-483. (SCI)
-
Huang,
Y.H., Lo, Y.H., Huang, W., Huang, C.Y.* (2012) Crystal
structure and DNA-binding mode of Klebsiella
pneumoniae primosomal PriB
protein. Genes Cells, 17, 837-849. (SCI); this article
was also selected in World Biomedical Frontiers (2013) Infection
and Immunity, 11, 17 (ISSN 2328-0166).
-
Lin,
H.H., Huang, C.Y.* (2012) Characterization of flavonol
inhibition of DnaB helicase: real-time monitoring, structural
modeling, and proposed mechanism. J. Biomed. Biotechnol.,
2012, 735368. (SCI)
-
Huang,
Y.H., Huang, C.Y.* (2012) Characterization
of a single-stranded DNA binding protein from Klebsiella
pneumoniae:
mutation at either
Arg73 or Ser76 causes a less cooperative
complex on DNA. Genes
Cells, 17, 146-157. (SCI)
-
Huang,
Y.H., Lin, H.H., Huang, C.Y.* (2012) A
single residue determines the cooperative binding property of a
primosomal DNA replication protein, PriB, to single-stranded DNA. Biosci.
Biotechnol. Biochem., 76, 1110-1115. (SCI)
-
Huang,
C.Y.* (2012) Determination
of the binding site-size of the protein-DNA complex by use of the
electrophoretic mobility shift assay,
an invited review as a
book chapter:
Stoichiometry and Research - The Importance of Quantity in Biomedicine
(ISBN 978-953-51-0198-7), Chapter
10, pp.
235-242, edited
by Innocenti, A., InTech Press Inc.
-
Ho,
Y.Y., Hsieh, H.C., Huang, C.Y.* (2011) Biochemical
characterization of allantoinase from Escherichia
coli BL21. Protein
J., 30, 384-394. (SCI)
-
Huang,
Y.H., Lee, Y.L., Huang, C.Y.* (2011) Characterization of
a single-stranded DNA binding protein from Salmonella enterica
serovar Typhimurium LT2. Protein J., 30, 102-108. (SCI)
-
Hsieh,
H.C., Huang, C.Y.* (2011) Identification of a novel
protein, PriB, in Klebsiella pneumoniae. Biochem.
Biophys. Res. Commun.,
404, 546-551. (SCI)
-
Chen,
C.C., Huang, C.Y.* (2011) Inhibition of Klebsiella
pneumoniae DnaB helicase by the flavonol galangin. Protein
J., 30, 59-65. (SCI)
-
Jan,
H.C., Lee, Y.L., Huang, C.Y.* (2011) Characterization of
a single-stranded DNA-binding protein from Pseudomonas aeruginosa
PAO1. Protein J., 30, 20-26. (SCI)
-
Wang,
C.C., Tsau, H.W., Chen, W.T., Huang, C.Y.* (2010)
Identification and characterization of a putative dihydroorotase,
KPN01074, from Klebsiella pneumoniae. Protein J.,
29, 445-452. (SCI)
-
Lo,
Y.H., Tsai, K.L., Sun, Y.J., Chen, W.T., Huang, C.Y.,
Hsiao, C.D. (2009)
The crystal
structure of a replicative hexameric helicase DnaC and its complex
with single-stranded DNA. Nucleic
Acids Res.,
37, 804-814. (SCI).
-
Huang,
C.Y., Hsu, C.C., Chen, M.C., Yang, Y.S. (2009) Effect of metal
binding and posttranslational lysine carboxylation on the activity of
recombinant hydantoinase. J. Biol. Inorg. Chem., 14,
111-121. (SCI)
-
Huang,
C.Y.*, Chang, Y.W., Chen, W.T. (2008) Crystal structure of the
N-terminal domain of Geobacillus kaustophilus HTA426 DnaD
protein. Biochem.
Biophys. Res. Commun., 375, 220-224. (SCI)
-
Tsai,
K.L., Sun, Y.J., Huang, C.Y., Yang, J.Y., Hung, M.C.,
Hsiao, C.D. (2007) Crystal structure of the human FOXO3a-DBD/DNA
complex suggests the effects of post-translational modification. Nucleic
Acids Res., 35, 6984-6994. (SCI)
-
Huang,
C.Y.,
Hsu, C.H., Sun, Y.J., Wu, H.N., Hsiao, C.D. (2006) Complexed crystal
structure of replication restart primosome protein PriB reveals a
novel single-stranded DNA binding mode. Nucleic Acids Res.,
34, 3878-3886. (SCI)
-
Tsai,
K.L., Huang, C.Y., Chang, C.H., Sun, Y.J., Chuang, W.J.,
Hsiao, C.D. (2006) Crystal structure of the human FOXK1a/DNA complex
and its implications on the diverse binding specificity of winged
helix/forkhead proteins. J. Biol. Chem., 281,
17400-17409. (SCI)
-
Huang,
C.Y.,
Yang, Y.S. (2005) Discovery of a novel N-iminylamidase activity:
substrate specificity, chemicoselectivity and catalytic mechanism. Protein
Expr. Purif. 40, 203-11. (SCI)
-
Liu,
J.H., Chang, T.W., Huang, C.Y., Chen, S.U., Wu, H.N.,
Chang, M.C., Hsiao, C.D. (2004) Crystal structure of PriB, a
primosomal DNA replication protein of Escherichia coli. J.
Biol. Chem. 279, 50465-71. (SCI)
-
Huang,
C.Y.,
Chao, Y.P., Yang, Y.S. (2003) Purification of industrial hydantoinase
in one chromatographic step without affinity tag. Protein Expr.
Purif. 30, 134-139. (SCI)
-
Huang,
C.Y.,
Chiang, S.K., Yang, Y.S., Sun, Y.J. (2003) Crystallization and
preliminary X-ray diffraction analysis of thermophilic imidase from
pig liver. Acta Crystallogr. D. Biol. Crystallogr. 59,
943-945. (SCI)
-
Huang,
C.Y.,
Yang, Y.S. (2003) A novel cold-adapted imidase from fish Oreochromis
niloticus that catalyzes hydrolysis of maleimide. Biochem.
Biophys. Res. Commun. 312, 467-472. (SCI)
-
Huang,
C.Y., Yang, Y.S. (2002) The role of metal on imide hydrolysis:
metal content and pH profiles of metal ion-replaced mammalian imidase.
Biochem. Biophys. Res. Commun. 297, 1027-1032. (SCI)
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